Abstract Number: 35

The use of multi-photon microscopy as a tool for studying elastin and elastin-related disorders in human skin

P.L. Tong , J. Qin , B. Roediger , P.M. Lowe , , T. Leecy , P. Mrass , W. Weninger, N.K. Haass

Meeting: 2012 Dermcoll

Session Information

Date: -

Session Title: Free Papers Session

Session Time: -

Multi-photon excitation laser scanning microscopy (MPM), relying on simultaneous absorption of two or more photons by a molecule, has been proposed as a novel diagnostic tool in biomedical imaging. Although applied in experimental skin biology, it has potential applications in clinical research due to the high penetration depth of infrared laser light. Current imaging modalities include dermoscopy, ultrasound, refl ectance confocal microscopy (RCM) and optical coherence tomography. Elastic fi bres, composed of two proteins elastin and fi brillin, form a network throughout the dermis and provide the skin its ability to stretch and recoil. Sun-damaged skin is characterised by its leathery appearance with deep furrows, telangiectasia and changes in skin pigmentation. Accumu- lation of abnormal elastic material, termed solar elastosis, is a prominent histological fi nding in photo-damaged skin. Using MPM, we demonstrate with histological resolution the morphological differences between normal and sundamaged skin in human skin samples. We fi nd that there is a marked difference in both thickness and shape of elastic fi bres in photo-damaged skin compared to non-exposed skin. Furthermore, changes in these fi bres were demonstrated in other elastin-related disorders. Of note, in a case of Pseudoxanthoma elasticum (PXE)-like syndrome we showed extensive abnormal, curled and frayed elastic fi bres in the reticular dermis that is characteristic of PXE using MPM.

Similar to the evolution of RCM from a research setting to
actual clinical use, it is anticipated that this technology may
become available as an adjunct to clinical diagnosis and monitoring of many skin conditions by providing real-time
diagnostics in a non-invasive manner.